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Title Alterations of Gut Microbiota Associated with Distinct Allergic Phenotypes: Big Data from An Asian Longitudinal Birth Cohort Study |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Translational Microbiome Studies |
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Main Author Preecha Patumcharoenpol1 |
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Presenting Author Preecha Patumcharoenpol1 Anchalee Senavonge2 |
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Co-Author Anchalee Senavonge2 Massalin Nakphaichit3 Pantipa Chatchatee2 Wanwipa Vongsangnak4 5 Sunee Nitisinprasert3 Sittiruk Roytrakul6 Narissara Suratannon2 |
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Department / Institution / Country Interdisciplinary Graduate Program in Bioscience / Faculty of Science, Kasetsart University / Thailand (ไทย)1 Department of Pediatrics / Faculty of Medicine, Chulalongkorn University. / Thailand (ไทย)2 Department of Biotechnology / Faculty of Agro-Industry, Kasetsart University / Thailand (ไทย)3 Department of Zoology / Faculty of Science, Kasetsart University / Thailand (ไทย)4 Computational Biomodelling Laboratory for Agricultural Science and Technology (CBLAST) / Faculty of Science, Kasetsart University / Thailand (ไทย)5 Genome technology Research Unit / National Center for Genetic Engineering and Biotechnology (BIOTEC) / Thailand (ไทย)6 |
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Background and Rationale Allergic diseases usually originate in early life. The interaction between gut microbiota and host immune cells shapes the immune development and affects the manifestations of allergic phenotypes. Whether gut microbiota change is different among distinct allergic phenotypes has never been elucidated in a longitudinal birth cohort. |
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Objectives: Indicates the purpose of the study We aimed to determine the difference of gut microbial colonization in distinct allergic diseases in infants age 9-12 months who participated in the longitudinal birth cohort. |
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Methodology: Describe pertinent experimental procedures A longitudinal population-based birth cohort study was conducted in Bangkok, Thailand. Diagnosis of allergic diseases was confirmed by allergists. The gut microbiome from stool of allergic infants and matched healthy controls (1:1) were analyzed using 16s amplicon sequencing method. |
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Results: Summarize the results of the research Of the 336 subjects, the incidence of allergic diseases was 12.5%. Three atopic phenotypes including atopic dermatitis (AD) 58.8%, food allergy (FA) 17.7% and subjects who have both AD and FA (AD/FA) 23.5% were identified. Shannon index revealed that microbial diversity of allergic infants was not significant different compared to controls. However, the diversity index of FA samples was the lowest at 2.08 whereas the others were in range of 2.32-2.38. Microbial compositions between four groups were mostly similar. Interestingly, the amount of family Erysipelotrichaceae in AD and AD/FA groups was significantly higher than controls (p<0.05). Erysipelotrichaceae was suggested to correlate with inflammation in human. The relative abundance of Bacteroidaceae and Enterobacteriaceae in FA and AD/FA group was slightly higher than controls, while Bifidobacteriaceae was oppositely presented. |
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Conclusions: State the main conclusions The change of gut microbiota of infants related to allergic diseases. Different allergic phenotypes have impact on shaping of microbiome in infant. This finding serves for further study in longitudinal birth cohort. Understanding the dynamic of microbial colonization patterns in allergic diseases will lead to discovering a promising clinical target for allergy prevention and treatment. |