|
Title The Effects of 1-Kestose on Intestinal Microbiota in Dog |
|
Type Poster Presentation |
|
Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
|
Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
|
Main Author Mikako Shinohara1 |
|
Presenting Author Mikako Shinohara1 |
|
Co-Author Kaori Ide2 Takumi Tochio1 Akihito Endo3 Koji Nishifuji2 |
|
Department / Institution / Country Research and Development center / B Food Science Co., Ltd. / Japan (日本)1 Division of Animal Life Science, Graduate School of Agriculture, Tokyo University of Agriculture / Tokyo University of Agriculture and Technology / Japan (日本)2 Department of Food and Cosmetic Science, Faculty of Bioindustry / Tokyo University of Agriculture / Japan (日本)3 |
|
Background and Rationale 1-kestose (kestose) is an important prebiotic short-chain fructooligosaccharide (FOS) composed of one glucose and two fructose molecules. Previous intervention and in vivo studies demonstrated that kestose is a promising prebiotic for humans and others. |
|
Objectives: Indicates the purpose of the study The objective of this study was to study the effects of kestose on intestinal microbiota in dogs. |
|
Methodology: Describe pertinent experimental procedures Five adult beagle dogs were fed 2 g/head of kestose, once daily for 56 days. The dogs were also given the regular diet from day 0 unl day 84 (day 57-84, washing period). Fresh fecal samples collected at day 0, day 28, day 56 and day 84 were subjected to 16S rRNA gene metagenomic analysis and quantitative real-time PCR analysis. Fecal concentration of short-chain fatty acids (SCFA; acetate, propionate, butyrate and lactate) were also determined. |
|
Results: Summarize the results of the research Metagenomic analysis revealed that the predominant intestinal microbiota in dogs were the genus Lactobacillus. The composition of Bifidobacterium increased at day 28 and maintained at the high level by the end of the study period. Quantitative real-time PCR analysis revealed that the cell number of Bifidobacterium increased at day 28, while that of Clostridium perfringens fell below the detection limit. Moreover, measurement of SCFA showed significant increase of butyrate in fecal contents, while specific butyrate producers did not significantly increased. |
|
Conclusions: State the main conclusions Our findings suggest that oral administration of kestose increases beneficial microbes in gut and decreases possible enteric pathogens in dogs. Gut butyrate level, which is crucial for health of several animals, can be increased in dogs by administration of kestose. |