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Title Dietary 1-Kestose Improves the Cecal Microbiota Composition in Association with a Remarkable Increase in the Cecal Butyrate Content in Rats |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Hirano Katsuaki1 |
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Presenting Author Hirano Katsuaki1 |
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Co-Author Tochio Takumi1 Kitaura Yasuyuki2 Endo Akihito3 Koga Yasuyuki4 Shimomura Yoshiharu2 |
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Department / Institution / Country Research and Development center / B Food Science Co., Ltd. / Japan (日本)1 Graduate School of Bioagricultural Sciences / Nagoya University / Japan (日本)2 Faculty of Bioindustry / Tokyo University of Agriculture / Japan (日本)3 Laboratory for Infectious Diseases / Tokai University School of Medicine / Japan (日本)4 |
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Background and Rationale Functional food ingredients, including prebiotics, have been ardently developed for improving composition of gut microbiota. Fructooligosaccarides (FOS), including fructans, are the well researched and commercialized prebiotics. However, few studies have been conducted on the physiological effects of each component of FOS as prebiotics. 1-Kestose, a component of FOS, is composed of one glucose and two fructose molecules, and former studies suggested that the tri-saccharide is a key prebiotic component in short-chain FOS. |
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Objectives: Indicates the purpose of the study In the present study, we examined the somatic effects of 1-kestose and its responsible mechanisms. |
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Methodology: Describe pertinent experimental procedures We used 40 male Sprague-Dawley rats aged 8 weeks, and the rats were randomly allocated to five groups (n = 8 per group): control (0%), and 0.5-5.0% 1-kestose diet groups. To examine the physiological effect of 1-kestose, short-chain fatty acids, cecal microbiota and blood components (cholesterol, triglyceride, insulin and plasma glucose) were determined. |
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Results: Summarize the results of the research We found that dietary 1-kestose induced cecal hypertrophy and alterations in the cecal microbiota composition, including a marked increase in the cell number of Bifidobacterium spp: approximately 7,000-fold increase in Bifidobacterium spp by feeding of 5% 1-kestose diet. These alterations were associated with significant increases in acetate and lactate, and a marked increase in butyrate in cecal contents. Furthermore, dietary 1-kestose induced a significant decrease in serum insulin concentration in rats fed 2.5-5% 1-kestose diet. |
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Conclusions: State the main conclusions These results suggest a potential of 1-kestose to be a prebiotic for improving the metabolism regulated by insulin. |