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Title Gut Microbiota in Pregnant Women: Connection with Obese Status and Inflammatory Biomarkers. |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Translational Microbiome Studies |
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Main Author Maria Florencia Zacarias1 |
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Presenting Author Maria Florencia Zacarias1 |
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Co-Author María Carmen Collado2 Carlos Gómez-Gallego1 Heini Flinck3 Janne Aittoniemi3 Erika Isolauri4 5 Seppo Salminen1 |
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Department / Institution / Country Functional Foods Forum, Faculty of Medicine / University of Turku / Finland (Suomi)1 Biotechnology / Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC) / Spain (España)2 Clinical Microbiology / Firmlab Laboratories / Finland (Suomi)3 Pediatrics and Adolescent Medicine / Turku University Hospital / Finland (Suomi)4 Clinical Sciences, Faculty of Medicine / University of Turku / Finland (Suomi)5 |
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Background and Rationale Obesity has been linked with dysbiosis and low grade inflammation. In pregnancy is associated with increased risk of childhood obesity and other diseases. To study the microbiota composition during pregnancy and how it is affected by pregestational BMI (pre-BMI) and the inflammatory status of the mothers can be the first step to elucidate new probiotic interventions towards a healthier microbial ecosystem. |
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Objectives: Indicates the purpose of the study Our aim was to study microbiota composition in mothers at third trimester of pregnancy, and to identify inflammatory biomarkers-microbiota interactions and their potential role in assessing overweight or obese status. |
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Methodology: Describe pertinent experimental procedures Fifty-four pregnant women were classified according to pre-BMI as normoweight, overweight or obese. At third trimester, blood and faecal samples were collected and serum inflammatory biomarkers (hs_CRP, haptoglobin and suPAR) and gut microbiota composition (16S gene sequencing) were assessed. Bioinformatics and statistical analysis were used to analyze all data. |
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Results: Summarize the results of the research Higher Firmicutes levels and a higher ratio Firmicutes/Bacteroidetes were observed in the obese group and high hs_CRP and haptoglobin levels correlated with decreased microbiota diversity (Shannon index). All the inflammatory markers were positively associated with pre-BMI but only haptoglobin was significantly higher in overweight and obese mothers. A core of positive and negative correlations between pre-BMI, biomarkers and specific bacterial groups were found. Whereas hs_CRP and haptoglobin values were correlated with specific microbiota components such as Ruminococcus gnavus and Faecalibacterium, no significant correlations were found for suPAR. |
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Conclusions: State the main conclusions Microbiota composition at third trimester was affected by pre-BMI and the inflammatory status of the mothers. Haptoglobin and hs_CRP reflected overweight and related microbiota components but haptoglobin was a better biomarker for microbiota associated overweight. suPAR was not related to deviated microbiota profiles. Understanding the composition and specific alterations of microbiota throughout pregnancy and finding biomarkers that reflect these changes, is of great value for opening new research avenues and probiotic therapeutic approaches. |