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Title Resistant Starch Ameliorates Advanced Glycation Endproduct-Induced Albuminuria in A Mouse Model Of Type 2 Diabetes |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Translational Microbiome Studies |
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Main Author Melinda Coughlan1 |
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Presenting Author Melinda Coughlan1 |
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Co-Author Sih Min Tan1 Runa Lindblom1 Vicki Thallas-Bonke1 Mark Cooper1 Matthew Snelson |
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Department / Institution / Country Diabetes / Monash University / Australia1 |
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Background and Rationale Excessive intake of dietary advanced glycation endproducts (AGEs) contributes to chronic renal injury. Recent research implicates gut dysbiosis in the progression of diabetic nephropathy, however, the role of dietary AGEs in gut dysbiosis and renal injury remains unexplored. |
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Objectives: Indicates the purpose of the study This study investigated whether excess consumption of dietary AGEs cause gut dysbiosis, exacerbating renal injury in a mouse model of type 2 diabetes. A secondary aim was to elucidate whether resistant starch (RS) supplementation is protective against diabetic nephropathy via altering gut homeostasis. |
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Methodology: Describe pertinent experimental procedures Six week old diabetic mice (db/db) and age-matched non-diabetic control mice (db/m) were randomised (n=12/group) to receive a low AGE (unbaked rodent chow) or a high AGE diet (baked at 160°C for 1 hour), with or without 25% RS for 10 weeks. All diets were isocaloric. 24-hour urine was collected for the assessment of albuminuria. Intestinal permeability was assessed in vivo by the clearance of FITC-labelled dextran. Cecal contents were collected and the V3-V4 region of the bacterial 16S rRNA gene were sequenced. |
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Results: Summarize the results of the research The high AGE diet exacerbated albuminuria in db/db mice, and this was attenuated by RS. Db/db mice had increased gut permeability compared to db/m mice. Furthermore, the high AGE diet increased gut permeability of db/db mice, an effect not observed in RS-fed db/db mice. In db/db mice, a high AGE diet was associated with an increase in the Firmicutes/Bacteroidetes (F/B) ratio, which was ameliorated by supplementation with RS. |
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Conclusions: State the main conclusions A high AGE diet led to increased intestinal permeability, an increase in F/B ratio, and worsening albuminuria in db/db mice. RS was protective against high AGE induced albuminuria in db/db mice. These preliminary studies support the notion that dietary AGEs contribute to renal disease via alterations in gut homeostasis and suggest a potential role for RS as a renoprotective agent. |