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Title Lactobacillus Rhamnosus GG Consumption Characteristically Modulate Gut Microbial Composition of Healthy Japanese Subjects. A Randomized, Double-Blind, Placebo-Controlled Study |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Probiotic and Prebiotic Clinical Research, From Newborns to Elderly |
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Main Author Gaku Harata1 |
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Presenting Author Gaku Harata1 |
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Co-Author Kenji Miyazawa1 Kazutoyo Yoda1 Kazuyuki Yamazaki1 Fang He1 Masaru Hiramatsu1 Shujiro Okuda2 |
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Department / Institution / Country Technical Research / Takanashi milk product Co.Ltd. / Japan (日本)1 Graduate School of Medical and Dental Sciences / Niigata University / Japan (日本)2 |
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Background and Rationale Lactobacillus rhamnosus GG (LGG) is one of the well-researched probiotics in the world. This bacterium can promote digestive health, alleviate various physiological/or clinic disorders such as atopic eczema and gastroenterological diseases, and might reverse dysbiosis to restore gut mucosal homeostasis. However, the benefits of LGG to the healthy subjects remain largely unclear. |
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Objectives: Indicates the purpose of the study This study was to evaluate how LGG could affect intestinal environment and gut microbiota composition in healthy Japanese subjects. |
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Methodology: Describe pertinent experimental procedures Lyophilized LGG was orally administrated to total 89 of healthy Japanese subjects with the dosage of 1.4×1010 CFU/day in a randomized, double-blind, placebo-controlled trail. Before and after 4 weeks intervention, the faecal were collected from the tested subjects, and fecal microbiota was profiled using sequencing of 16S rRNA genes of faecal DNA. |
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Results: Summarize the results of the research Phylum Proteobacteria were significantly decreased (P=0.01) and tend to decrease family Enterobactericeae (P=0.06) after administration of LGG. Significant decreases in genus Clostridium (family Clostridiaceae) (P=0.04) and increase in Bacillus (P=0.01) were also found after intervention. However, no significant changes were observed in the placebo group. Interestingly, the same tendency was also found in female subject treated with LGG (n=34), the abundance of family Enterobactericeae were decreased (P=0.02) and genus Bacillus and Lactobacillus were increased significantly (P=0.01, P=0.05), while in male, genus Turicibacter and Roseburia were increased in man (n=11) after LGG consumption. |
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Conclusions: State the main conclusions These results indicate LGG might benefit to healthy subjects though modifying intestinal microbes characteristically. These findings suggest the prospective studies to investigate the LGG–microbiome interaction in human health and disease. |