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Title Levothyroxine Improves Throid Dysfunction Closely Related With Gut Microbiota and Its Metabolites |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Jinghua Wang1 |
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Presenting Author Jinghua Wang1 |
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Co-Author narae shin1 meng yang1 soo-kyoung lim1 hojun kim1 |
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Department / Institution / Country Department of Oriental Rehabilitation Medicine / Dongguk University / Korea, Rep. (대한민국)1 |
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Background and Rationale Many gut problems can suppress thyroid function and conversely low thyroid function induce gut inflammation and enlargement of gut permeability. Thus trillions of commensal bacteria in gut exerts essential roles in keeping homeostasis may including thyroid hormones. |
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Objectives: Indicates the purpose of the study The present study aimed to investigate the relationship between hypothyroidism and gut microbiota and mine the beneficial bacterial species as a novel therapy for ameliorating thyroid problems and related complications. |
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Methodology: Describe pertinent experimental procedures Hypothyroidism was induced by propylthiouracil (PTU, 0.1% in drinking water) for 4 weeks in rats. Meanwhile administration group was given levothyroxine (200 ug/kg, i.p.) injection once a day for last 2 weeks. Fecal 16s rDNAs were sequenced by Next-generation Sequencing (Ion Torrent) for analyzing gut microbiota. |
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Results: Summarize the results of the research Hypothyroidism model was established successfully as evidenced by notably low serum T4 and high serum TSH in PTU treatment group. Levothyroxine treatment significantly ameliorated the change of serum T4 and TSH hormones. Interestingly, PTU markedly reduced the endotoxin level both in fecal and serum as compared to other groups in accordance with reduction of gram-negative bacteria abundance and elevation of intestinal alkaline phosphatase (a major defense regulated by thyroid hormone against lipopolysaccharide) concentration. Besides, it was noticeably found that a holistic profiling change of gut microbiota between PTU induced hypothyroidism group and the other groups, but not gram-positive bacteria. In detail, PTU induced hypothyroidism evidently increased the Bacteroides acidifaciens, Clostridiales, Rc4-4 and decreased the Dorea. Whereas, levothyroxine distinctly restored these bacteria following the recovery of thyroid function. |
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Conclusions: State the main conclusions Taken together, these results demonstrated that levothyroxine improves PTU induced hypothyroidism through maintaining stability of endotoxin and alkaline phosphatase intimately associated with gut microbiota alteration. |