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Title Efficiency of B–Glucan Extract From Auricularia Auricula-Judae And Schizophyllum Commune in Thailand on the Recovery of Cell Lines Treated With Anticancer Drug and Cytotoxicity in Cell Lines |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Probiotic and Prebiotic Safety |
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Main Author Korawan Chakree1 |
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Presenting Author Korawan Chakree1 |
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Co-Author Sukanya Settharaksa2 Anchana Ampai1 Santad Wichienchot1 |
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Department / Institution / Country Interdisciplinary Graduate School of Nutraceutical and Functional Food / Interdisciplinary Graduate School of Nutraceutical and Functional Food, Prince of Songkla University / Thailand (ไทย)1 Sino-Thai Traditional Medicine Research Center / Sino-Thai Traditional Medicine Research Center, Faculty of Pharmacy, Rangsit University / Thailand (ไทย)2 |
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Background and Rationale Anti-cancer drug, cisplatin is a types of cancer treatments that are well-known and effective. However, the cancer treatment with anticancer drugs results in side effects such as nausea and vomit, causes of nervous system, kidney and bone marrow, and affect eating and nutritional status of patients. b-glucan, a prebiotic derived from mushrooms, plays an important role in health as it enhances or improves the body’s immune system including cancer patients. |
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Objectives: Indicates the purpose of the study This research aims to evaluate the cytotoxicity of b-glucan extract from two edible mushrooms in Thailand on normal kidney cell lines and the recovery of cell lines treated with cisplatin. Two edible mushrooms, Auricularia auricula-judae and Schizophyllum commune, in Thailand were selected to extract the b-glucan and then they are used in the experiments. |
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Methodology: Describe pertinent experimental procedures b-glucan from A. auricula-judae and S. commune were extracted at high pressure and temperature of 130 °C for 20 min. b-glucan extracts were purified by purified by ultrafiltration membrane and then freezed dry. The molecular mass of b-glucan extracts was analyzed by High Perfomance Size Exclusion Chromatography (HPSEC). The efficiency of b-glucan extracts on the recovery of cell lines treated with anticancer drug and cytotoxicity in HEK 293 and LLC-RK1cell lines were studied. |
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Results: Summarize the results of the research b-glucan in extracted sample from A. auricula-judae and S. commune were 24.50±1.30 %w/w and 25.54±0.55 %w/w while commercial b-glucan from commercial yeast was 46.21±0.37 %w/w. For the cytotoxicity, the cytotoxicity on HEK 293 and LLC-RK1 cell lines increased with increase in concentration of samples but b-glucan could not recover the cisplatin drug treated cell lines. |
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Conclusions: State the main conclusions b-glucan extracts from A. auricula-judae and S. commune has less cytotoxic with kidney normal cell lines. However, they still cannot recovery the damaged cells, they might should be a supplement or ingredient. |