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Title In Vitro Enrichment: A Novel Method for Formulating Synergistic Synbiotics |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Influence of Probiotics and Prebiotics on Diet, Nutrition and Drug Metabolism |
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Main Author Car Reen Kok1 |
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Presenting Author Car Reen Kok1 |
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Co-Author Robert Hutkins1 |
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Department / Institution / Country Food Science & Technology / University of Nebraska-Lincoln / United States1 |
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Background and Rationale One strategy to modulate the gastrointestinal microbiota and improve gut and systemic health is by consumption of probiotics, prebiotics, or synbiotics. The latter can be formulated in one of two ways. Complementary synbiotics are comprised of combinations of probiotics and prebiotics, with each component functioning independently. In contrast, synergistic synbiotics contain probiotic strains that consume and are stimulated by the cognate prebiotic. Synergistic synbiotics may, therefore, have an ecological advantage by enhancing the ability of the probiotic to compete in the gastrointestinal tract. |
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Objectives: Indicates the purpose of the study The goal of this study was to develop a rational method for selecting putative strains that would have synergistic properties. |
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Methodology: Describe pertinent experimental procedures Our approach was to use stepwise in vitro fermentations to enrich for strains able to use the prebiotic, xylooligosaccharide (XOS). The in vitro enrichment (IVE) method was started by addition of a fecal slurry to fermentation vessels containing XOS. Every 24 hours, portions were transferred into fresh medium, until about 20 generations had been achieved. Samples were plated on Bifidobacterium selection agar, and XOS-fermenting isolates were obtained and identified by 16S rRNA sequencing. Genus- and species-specific real-time (RT)-PCR was used to quantify bifidobacteria. |
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Results: Summarize the results of the research One isolate, Bifidobacterium longum subsp. longum CR15 was re-introduced into nine fermentation vessels, each containing XOS and different fecal samples. After approximately 20 generations, the CR15 strain was recovered in six vessels and was the dominant Bifidobacterium strain in three. Genome sequencing of B. longum CR15 revealed the presence of enzymes belonging to two different glycosyl hydrolases families crucial for XOS utilization. |
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Conclusions: State the main conclusions In conclusion, the IVE method is a quick, convenient method for isolating potential synergistic probiotic strains capable of fermenting XOS or other specific prebiotic substrates. These in vitro results also suggest that the resident microbiota still has a profound influence on the establishment of particular strains, even when synergistic synbiotics are used. |