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Title Effect of Vitamins B2 and C on the Development of Mucositis |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Influence of Probiotics and Prebiotics on Diet, Nutrition and Drug Metabolism |
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Main Author Ana Rita Ferreira1 |
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Presenting Author Ana Rita Ferreira1 |
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Co-Author Wim J.E. Tissing 2 Gabriela Bravo-Ruiseco1 Folkert Kuipers2 Hermie J.M. Harmsen 1 |
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Department / Institution / Country Department of Medical Microbiology / University Medical Groningen Center / Netherlands1 Department of Pedriatics / University Medical Groningen Center / Netherlands2 |
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Background and Rationale Chemotherapy-induced mucositis is a severe side-effect of anti-cancer treatment. This inflammatory disorder induces alterations in the composition of the gut microbiota, including a decrease in anaerobic bacteria. Redox active compounds, such as vitamins B2 and C, were shown to reduce inflammation in the gut and to enhance the growth of anaerobic bacteria. |
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Objectives: Indicates the purpose of the study We aim at investigating the effects vitamins B2 and C on the development of mucositis and on the composition of the gut microbiota in the methotrexate (MTX)-induced mucositis rat model. |
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Methodology: Describe pertinent experimental procedures MTX-induced mucositis rats were daily gavaged with vitamins B2 and C, alone or in conjugation, and MTX (45 mg/Kg) or saline solution (control) was administrated via intravenous injection. The severity of mucositis was determined by food intake, body weight and plasma citrulline and the gut microbiota composition was characterized by 16S rRNA sequencing. A combination of different concentrations of vitamins was added to bacterial cultures isolated from rats and their grow assessed. |
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Results: Summarize the results of the research Vitamin C supplementation (250mg/Kg/day) in rats attenuated the severity of mucositis reflected by changes in body weight, food intake and plasma citrulline. A dose-depended effect of vitamin B2 was found, as higher doses (60mg/Kg/day) have a negative impact on mucositis whereas lower doses (10 mg/Kg/day) have no effect. Anaerobic bacteria Blautia coccoides and Roseburia intestinalis significantly grew better in the presence of oxygen, when a combination of 0.5 mM or 1 mM of both vitamins is added to the culture. |
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Conclusions: State the main conclusions Vitamin C ameliorates mucositis in our rat model. Vitamins B2 and C enhance the growth of anaerobic bacteria under oxidative stress. |