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Title The Effect of Non-Ionic Detergent on the Growth of Faecalibacterium Prasusnitzii in Vitro |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Metabolic By-products of Probiotic Strains |
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Main Author Lu Wang1 |
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Presenting Author Lu Wang1 |
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Co-Author Gabriela Bravo-Ruseco1 Rieks Hoekman1 Hermie Harmsen1 |
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Department / Institution / Country Medical Microbiology / University Medical Center Groningen / Netherlands1 |
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Background and Rationale Faecalibacterium prausnitzii is considered as a potential probiotic because of its anti-inflammatory properties. Its delayed colonization in childhood is correlated with development of auto-immune diseases, such as allergy and type 1 diabetes. Recently, hygiene hypothesis is believed to affect the infant gut microbiota composition. Parents tend to clean their baby frequently nowadays, which may cause an overexposure to non-ionic detergent. Previous findings show that F. prausnitzii often co-occurs with Bacteroides thetaiotaomicron. Therefore, we hypothesize that an overuse of detergent could affect the colonization of F. prausnitzii and that B. thetaiotaomicron could help to overcome this effect. |
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Objectives: Indicates the purpose of the study To investigate if the detergent could affect the colonization of F. prausnitzii and the interaction between different species and to explain the mechanism by discussing the integrity and function of bacteria cell membrane. |
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Methodology: Describe pertinent experimental procedures The experiments were performed by growing F. prausnitzii and B. thetaiotaomicron separately and together in YCFAG medium (with and without acetate) with different concentrations (0 to120 µg/ml) of Triton X-100 for 48 hours. Samples hereof were analyzed with fluorescence in situ hybridization (FISH) to enumerate the subpopulations. Microbial fuel cell (MFC) and transmission electron microscopy (TEM) were employed to investigate the cell membrane integrity and function. |
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Results: Summarize the results of the research We found that the growth of F. prausnitzii was inhibited by Triton X-100. FISH counting results showed B. thetaiotaomicron stimulated the growth of F. prausnitzii independent of the presence of Triton X-100 and acetate. TEM and MFC results showed the thickness of bacteria cell envelope and the current produced by F. prausnitzii were affected. |
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Conclusions: State the main conclusions Our study shows Triton X-100 inhibits the growth of F. prausnitzii maybe because it destroys the integrity and function of cell membrane, the mechanism still needs to be investigated further. B. thetaiotaomicron could help to overcome this effect. |