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Title Considering Antibiotic Resistance Profiles of Lactobacillus Strains Isolated from the Female Gential Tract of African Women, Relevant to their Development as Probiotic Strains |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Probiotic and Prebiotic Safety |
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Main Author Anna-Ursula Happel1 |
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Presenting Author Anna-Ursula Happel1 |
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Co-Author Chambrez Zauchenberger1 Hoyam Gamielden1 Shameem Z. Jaumdally1 2 Shaun Barnabas1 Heather Jaspan1 4 Jo-Ann Passmore1 2 3 |
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Department / Institution / Country Pathology / University of Cape Town / South Africa1 Immunology / DST-NRF Centre of Excellence in HIV Prevention / South Africa2 Immunology / National Health Laboratory Service / South Africa3 Seattle Children’s Research Institute / University of Washington / United States4 |
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Background and Rationale Antibiotic resistance is a threat to global health. The antibiotic resistance profiles of Lactobacillus spp., a significant component of microbiota at various body sites, are of major interest. |
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Objectives: Indicates the purpose of the study The susceptibility of potentially probiotic Lactobacillus strains isolated from female genital tracts and commercial vaginal probiotics, used to treat bacterial vaginosis (BV), to metronidazole and clindamycin, the standard treatment for BV, as well as to a selection of inhibitors of protein-, cell wall-, membrane- and DNA synthesis commonly used in South Africa was determined. |
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Methodology: Describe pertinent experimental procedures The susceptibility of Lactobacillus strains isolated from probiotics for vaginal health (L. reuteri [n=3], L. rhamnosus [n=8], L. acidophilus [n=3]) and from cervico-vaginal fluid from healthy South African women (L. crispatus [n=10], L. gasseri [n=9], L. jensenii [n=18], L. vaginalis [n=8], L. mucosae [n=12]) to metronidazole and clindamycin was determined using the disc diffusion method. The antibiotic susceptibility profile was determined for a subset of 20 selected strains to 21 antibiotics: protein synthesis inhibitors (chloramphenicol, linezolid, tetracylince, tigecycline, clindamycin, erythromycin, streptomycin, gentamycin, quinupristin/dalfipristin), cell wall/membrane inhibitors (penicillin, vancomycin, nitrofurantoin, oxacillin, ampicillin, daptomycin), DNA synthesis inhibitors (moxifloxacin, ciprofloxacin, levofloxacin) using SensititreTM plates. |
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Results: Summarize the results of the research While all Lactobacillus isolates were resistant to metronidazole, the clinical L. vaginalis strains were the most susceptible to clindamycin, followed by L. jensenii (clinical), L. mucosae (clinical), L. reuteri (probiotic), L. rhamnosus (probiotic), L. crispatus (clinical), L. acidophilus (probiotic), and lastly L. gasseri (clinical). The strains isolated from commercial probiotics were the least susceptible to the 21 antibiotics tested while clinical L. gasseri isolates showed the most susceptible patterns. |
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Conclusions: State the main conclusions These antibiotic susceptibility patterns for common probiotic and clinical Lactobacillus isolates highlight the need to determine the resistance mechanisms that may influence their utility in probiotic BV treatment. Specific antibiotic resistance determinants carried on mobile genetic elements constitute a potential reservoir of drug resistance for genital tract pathogens. |