|
Title Compromised Gut Microbiota At Birth |
|
Type Poster Presentation |
|
Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
|
Topic Translational Microbiome Studies |
|
Main Author Wei Wei Thwe Khine1 7 |
|
Presenting Author Wei Wei Thwe Khine1 7 |
|
Co-Author Christophe Lay2 3 Claudia Chi4 Mahesh Choolani4 Jan Knol5 6 Seppo Salminen7 Yuan Kun Lee1 |
|
Department / Institution / Country Microbiology and Immunology / National University of Singapore / Singapore1 Research and Innovation / Danone Nutricia Research / Singapore2 Paediatrics / National University Hospital / Singapore3 Obstetrics and Gynaecology / National University Hospital / Singapore4 Research and Innovation / Danone Nutricia Research / Netherlands5 Wageningen University / Wageningen University / Netherlands6 Food Development, Functional Foods Forum, Faculty of Medicine / University of Turku / Finland (Suomi)7 |
|
Background and Rationale Background and Rationale: A compromised gut microbiota in early life has been associated with the development of immune and metabolic disorders. |
|
Objectives: Indicates the purpose of the study Objectives: The objective of this study was to determine the effect of mode of delivery and intrapartum antibiotic prophylaxis (IAP) on the maturation of the infant gut microbiota. |
|
Methodology: Describe pertinent experimental procedures Methodology: Three groups of healthy term-born infants were recruited from the National University Hospital (Singapore): (1) Infants born vaginally (VD, n=21); (2) Infants born vaginally whose mothers received IAP (IAP, n=22); and (3) Infants born by C-section (CS, n=25). Stool samples (n=246 samples) were collected at birth, day 7 and, 1 and 3 months. The composition of the gut microbiota was determined by 16S rRNA sequencing.
|
|
Results: Summarize the results of the research Results: A delayed colonization by Bifidobacterium was observed in IAP and CS but not in VD born infants. This was statistically significant at day 7 (VD vs IAP, p=0.020; VD vs CS, p=0.003) and 1 month (VD vs IAP, p=0.003; VD vs CS, p=0.015). In contrast to infants born vaginally (with and without IAP exposure), CS born infants featured a delayed colonization by Bacteroides from day 7 (CS vs VD, p<0.0001; CS vs IAP, p=0.007) till 3 months (CS vs VD, p=0.003; CS vs IAP, p=0.007). The relative abundance of Enterobacteriaceae was higher in IAP and CS compared to VD born infants at day 7 (IAP vs VD, p=0.007 and CS vs VD, p=0.037) and 1 month (IAP vs VD, p=0.031 and CS vs VD, p=0.049). A negative correlation between Bifidobacterium and Enterobacteriaceae was observed at day 7 and 1 month. The species diversity was higher in VD compared to IAP and CS at day 7 (p=0.007 and p=0.001). |
|
Conclusions: State the main conclusions Conclusions: Intrapartum Antibiotic Prophylaxis and C-section impair the early microbial colonization and this may represent a risk factor for the development of immune/ metabolic disorders.
|