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Title Intranasal Lactobacillus Rhamnosus GG Ameliorates Airway Hyperreactivity and Allergic Airway Inflammation in Mice |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Irina Spacova1 2 |
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Presenting Author Irina Spacova1 2 |
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Co-Author Mariya Petrova1 2 Astrid Fremau2 Lore Pollaris4 Jeroen Vanoirbeek4 Jan L. Ceuppens3 Sven Seys3 Sarah Lebeer1 2 |
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Department / Institution / Country Department of Bioscience Engineering / University of Antwerp / Belgium1 Department of Microbial and Molecular Systems / KU Leuven / Belgium2 Department of Microbiology and Immunology / KU Leuven / Belgium3 Department of Public Health and Primary Care / KU Leuven / Belgium4 |
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Background and Rationale Probiotic bacteria have long shown potential for the attenuation of allergy-related conditions. Their intranasal administration represents a promising alternative delivery method in the context of airway inflammatory diseases. |
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Objectives: Indicates the purpose of the study The goal of this project was to explore the potential of preventive intranasal probiotic instillations using the model probiotic strains Lactobacillus rhamnosus GG and L. rhamnosus GR-1 in an experimental allergic asthma model in mice. |
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Methodology: Describe pertinent experimental procedures L. rhamnosus GG and L. rhamnosus GR-1 were intranasally administered in mice for two weeks. Intranasal colonization and translocation of the bacteria was assessed by plating nasal mucosa, fecal and lymph node samples on selective medium. Fluorescent strains of L. rhamnosus were used to study their binding to murine airway epithelial and immune cells in vitro. After the two-week L. rhamnosus treatment, allergic asthma was induced in mice with intranasal instillations of birch pollen extract, and effects of the treatment were analyzed based on antibody concentrations in blood, inflammatory cell counts in the lungs, lung Th2 cytokine levels and airway hyperreactivity. |
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Results: Summarize the results of the research Intranasal L. rhamnosus administration was demonstrated to be innocous and transient colonization of the nasal passages was observed. L. rhamnosus GG demonstrated superior binding to airway epithelial and immune cells in vitro compared to L. rhamnosus GR-1. In the murine asthma model, L. rhamnosus GG instillations also significantly reduced airway hyperreactivity and allergic airway inflammation, reflected in less pronounced lung eosinophilia and decreased Th2 cytokine levels. Compared to L. rhamnosus GG, instranasal L. rhamnosus GR-1 had rather a neutral effect on allergic airway inflammation, although a tendency towards attenuated airway hyperreactivity was also observed. |
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Conclusions: State the main conclusions Intranasal instillation of L. rhamnosus GG is a feasible administration approach in the treatment of airway allergic disease. It results in amelioration of the main hallmarks of allergic asthma in our mouse model, although validation in human clinical trials is still warranted. |