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Title Enhancement of the Anti-Inflammatory Activity of Lactobacillus Plantarum Strains by Optimization of Their Culture Condition |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Takayuki Toshimitsu1 |
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Presenting Author Takayuki Toshimitsu1 |
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Co-Author Satoru Ozaki1 Junko Mochizuki1 Keisuke Furuichi1 Yukio Asami1 |
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Department / Institution / Country Applied Microbiology Research Department / Food Microbiology Research Laboratories, Division of Research and Development, Meiji Co., Ltd. / Japan (日本)1 |
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Background and Rationale Background and Rationale: Recent reports suggest that culture conditions of lactic acid bacteria (LAB) affect their immunomodulatory properties. The anti-inflammatory activity of LAB is deduced to be an essential mechanism of their anti-metabolic disorder effects. Studies on the health-promoting effects of LAB are numerous, but few have comprehensively investigated the relationship between LAB functionality and their culture conditions. |
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Objectives: Indicates the purpose of the study Objectives: In the present study, we verified the effect of differences in culture conditions on Lactobacillus plantarum OLL2712 (OLL2712) functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. |
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Methodology: Describe pertinent experimental procedures Methodology: We measured cytokine productions in murine immune cells treated with OLL2712 cells prepared under various culture conditions. We also verified the preventive effects of OLL2712 cells at different culture conditions against inflammation and metabolic disorders in obese and diabetic mice. |
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Results: Summarize the results of the research Results: The results showed that IL-10-inducing activity of OLL2712 cells differed dramatically between OLL2712 groups at different culture phases, and under different culture medium components, temperatures, and neutralizing pH. We demonstrated several culture conditions of OLL2712 for higher anti-inflammatory activity. In particular, exponential phase cells had much more IL-10-inducing activity than stationary phase cells, and adding decaglycerol mono-oleic acid ester further improved the IL-10-inducing activity. Similar phenomena were observed in another strain of L. plantarum. These differences in IL-10-inducing activity were linked to differences in the toll-like receptor (TLR) 2 stimulation activity. We also demonstrated functional differences by culture phases in vivo; OLL2712 cells at exponential phase had more anti-inflammatory activity and anti-metabolic disorder effects on obese and diabetic mice than those at stationary phase. |
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Conclusions: State the main conclusions Conclusions: We confirmed that the functionality of OLL2712 cells differed by their culture conditions in vitro and in vivo. Our results suggest that culture conditions of LAB affect the anti-inflammatory activity, and optimization of culture conditions is quite important for their health-promoting functionality. |