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Title Supplementation with Prebiotic Inulin-Type Fructans on Host Metabolism and Gut Microbiota: The First Randomized Double-Blind Placebo-Controlled Trial in China |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Probiotic and Prebiotic Clinical Research, From Newborns to Elderly |
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Main Author Feitong Liu1 2 3 |
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Presenting Author Feitong Liu1 2 3 |
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Co-Author Yuemei Luo1 2 Muxuan Chen1 Chongbin Wu1 Fan Wu1 Pan Li1 2 Jie Li1 Hongwei Zhou1 |
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Department / Institution / Country Division of Laboratory Medicine / Zhujiang Hospital, Southern Medical University / China (中国)1 Department of Environmental Health / School of Public Health, Southern Medical University / China (中国)2 Department of Food and Nutritional Sciences / School of Chemistry, Food and Pharmacy, The University of Reading / United Kingdom3 |
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Background and Rationale Background and rationale: The gut microbiota has been implicated in host lipid and glucose metabolism. Now recent findings suggest that prebiotic results in bacterial ecosystem shift. Whereas, a comprehensive characterization of this effect is quite inadequate in Chinese Population.
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Objectives: Indicates the purpose of the study Objectives: Here, the objective of the present study was to assess the consequences of supplementation with inulin-type fructans (ITF), fructo-oligosaccharides (FOS) and inulin, on the composition of gut microbiota and human metabolism. |
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Methodology: Describe pertinent experimental procedures Methods: A randomized double-blind placebo-controlled study was conducted with 135 healthy adults with FOS and inulin for 4 weeks (15g/d). Blood and fecal sampling, oral glucose tolerance test and anthropometry were performed before and after intervention. 16S rDNA microbiota profiling was applied to assess the composition of gut microbiota. Machine learning with random forest model was adopted to predict the response of OGTT following ITF prebiotics intervention. |
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Results: Summarize the results of the research Results: 4-week intake of ITF prebiotics, but not placebo, markedly increased abundance of Bifidobacterium with significant effects on overall microbial richness and diversity. Meanwhile, the manipulation of ITF on gut microbiota was also manifested in the decreased abundance of Roseburia, Phascolarctobacterium, Ruminococcaceae, Ruminococcus, Holdemania and Lachnospiraceae. Importantly, we did not observe any differences in host anthropometric, lipid metabolism, blood glucose metabolism, nor insulin sensitivity. In addition, using the baseline data of gut microbiota in random forest model, we built an index to successfully predict the response of OGTT after ITF intervention. |
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Conclusions: State the main conclusions Conclusions: 4-week supplementation of ITF indeed led to ecosystem-wide microbiota shifts, but this did not produce significant alteration in host lipid and glucose metabolism. While with the data of initial gut microbiota, we can predict the individualized response of OGTT after ITF intervention, which make potential practical value in nutritional strategy. |