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Title Prebiotic Properties of Oligosaccharide Produced from the Spent Coffee Grounds by Mannanase from Bacillus Sp. GA2 (1) |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
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Main Author Chayaporn Wongsiridetchai1 |
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Presenting Author Chayaporn Wongsiridetchai1 |
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Co-Author Sudathip Chantorn1 Thornthan Sawangwan2 Theppanya Charoenrat1 Prasert Wongwathanarat 1 |
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Department / Institution / Country Biotechnology / Thammasat University / Thailand (ไทย)1 Biotechnology / Ramkamhaeng University / Thailand (ไทย)2 |
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Background and Rationale Agricultural and agro-industry wastes such as sugarcane bagasse, straw, soybean grounds, and spent coffee grounds mostly contain lignocellulose as main component which are digested by lignocellulosic enzyme to produce oligosaccharide from cellulose and hemicellulose. These oligosaccharides can use as substrate in various industries such as energy, food, and animal feed industry. In addition, the important property of oligosaccharides is prebiotic substance. |
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Objectives: Indicates the purpose of the study The objectives of this research were to analyze type of oligosaccharide from spent coffee grounds (SCG) producing by Bacillus sp. GA2(1) mannanase by thin layer chromatography (TLC) and high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). In addition, prebiotic properties of oligosaccharide were evaluated as follow the growth of lactic acid bacteria (LAB). |
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Methodology: Describe pertinent experimental procedures Treated-SCGs were digested by mannanase from Bacillus sp. GA2(1) to produce the oligosaccharides. The products were analyzed type of sugar by TLC and HPAEC-PAD methods. In addition, the important property of oligosaccharide is prebiotic substance so the growth of Lactobacillus acidophilus, Lactobacillus casei and Lactobacillus plantarum cultivated with oligosaccharide products were examined compared with control and commercial prebiotics (Fructooligosaccharide; FOS). |
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Results: Summarize the results of the research The oligosaccharide products from SCGs digested by mannanase from Bacillus sp. GA2(1) are mannose, mannobiose, mannotriose and mannopentaose. The determination of prebiotic properties of oligosaccharide found these products can promote the growth of L. acidophilus, L. casei and L. plantarum as much as the commercial prebiotics (FOS). |
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Conclusions: State the main conclusions This study provided early evidence that oligosaccharides from SCGs digested by mannanase from Bacillus sp. GA2(1) are prebiotic substance, which can be alternative prebiotic for industry. |