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Title Alteration in the Gastric Microbiota and Its Restoration by Probiotics in Patients with Functional Dyspepsia |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Translational Microbiome Studies |
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Main Author Yasuhiro Koga1 |
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Presenting Author Yasuhiro Koga1 |
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Co-Author |
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Department / Institution / Country Department of Gastroenterology / Tokai University School of Medicine/ / Japan (日本)1 |
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Background and Rationale Little is known about the gastric microbiota or its role in the pathophysiology of functional dyspepsia (FD).
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Objectives: Indicates the purpose of the study This study was perfomed to comparatively analyze the gastric fluid (GF) microbiota between FD and healthy controls (HC), and to assess the effect of probiotics on the microbiota. |
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Methodology: Describe pertinent experimental procedures Twenty-four Japanese patients with FD who met the Rome III definition and 21 age- and gender-matched HC volunteers were enrolled. The FD patients had been treated with LG21, a probiotic strain. GF was sampled after an overnight fast using a nasogastric tube. Bile acids concentration was determined by ELISA. The V3-V4 region of 16S rRNA gene was amplified using bacterial DNA from the GF, and then about 30,000 high-quality amplicons per sample were grouped into operational taxonomic units for analyses. |
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Results: Summarize the results of the research The ratio of GF samples in which the bile acids was detectable was significantly greater in the FD than in the HC groups. In the bacterial composition analysis at the phylum level, the GF microbiota had a Bacteroidetes>Proteobacteria abundance and an absence of Acidobacteria in the FD group, in contrast, the GF microbiota had a Bacteroidetes<Proteobacteria abundance and the presence of Acidobacteria in the HC group. Probiotic therapy in patients with FD shifted the composition of the gastric fluid microbiota to that observed in the HC volunteers. |
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Conclusions: State the main conclusions Alteration in the GF microbiota was found in FD patients compared with HC volunteers. Reflux of the small intestinal contents, including bile acids and intestinal bacteria, to the stomach was suggested to induce a bacterial composition change and be involved in the pathophysiology underlying FD. Probiotics appear effective in the treatment of FD through the normalization of gastric microbiota. |