Abstract Preview
Abstract
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Title Gut-Adaptive Genes of Probiotic Lactobacillus Acidophilus NCFM during In Vivo Colonization |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Influence of Probiotics and Prebiotics on Diet, Nutrition and Drug Metabolism |
Authors
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Main Author Yong Jun Goh1 |
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Presenting Author Yong Jun Goh1 |
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Co-Author Todd Klaenhammer1 Rodolphe Barrangou1 |
Authors' Institution
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Department / Institution / Country Food, Bioprocessing and Nutrition Sciences / North Carolina State University / United States1 |
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Background and Rationale Lactobacillus acidophilus is the most ubiquitously formulated probiotic culture in the dairy and dietary supplement industries. Our genetic and in vitro functional studies have elucidated the probiotic mechanisms of L. acidophilus, though study is still lacking on how L. acidophilus adapts and competes in the gut environment in vivo. |
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Objectives: Indicates the purpose of the study To establish probiotic-associated genes and to uncover novel genes in L. acidophilus that are crucial to gut retention and immunomodulation in vivo, and to assess the impact of probiotic colonization on host cytokines and intestinal tight junction proteins expression. |
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Methodology: Describe pertinent experimental procedures Six germ-free 129S6/SvEv mice were each administered with 6x108 CFU of L. acidophilus NCFM (treatment group), euthanized post-1 week, and intestinal tissues (duodenum, jejunum, ileum, cecum and colon) were harvested for RNA isolation. A control group of 3 mice without NCFM treatment was included. Bacterial RNA-seq transcriptome analysis was performed to compare gene expression under in vivo versus in vitro growth conditions. In parallel, host transcriptional responses to NCFM treatment were examined by qRT-PCR analyses of tissue RNA. |
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Results: Summarize the results of the research The majority of upregulated genes in L. acidophilus NCFM during gut transit encode for carbohydrate metabolism (e.g. prebiotic sugar transporters, glycogen metabolism), amino acid and nucleotide metabolism, cell division, DNA repair, stress/bile defense, mucus-binding/surface adhesins, and numerous unknown proteins, indicating their crucial roles in nutrient acquisition and gut fitness. The presence of putative gut-adaptive genomic islands reflect the specialized adaptation of NCFM to the mammalian gut. In NCFM-colonized mice, significant down-regulation of IL-12b and the tight junction proteins OCLN and TJP1 in the small intestine indicate an anti-inflammatory response and more stabilized intestinal barrier integrity conferred by interaction of NCFM with the host epithelium. |
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Conclusions: State the main conclusions This study identified gut-adaptive genes in L. acidophilus, and provided mechanistic insights in the survival and health-promoting activities of L. acidophilus and likely other probiotic lactobacilli in their hosts. |
Requires Audio or Video system for Presentation?: No
