Abstract Preview
Abstract
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Title Bio-Prospecting Of Vitamin B2 Producing Lactobacillus Strains of Indigenous Origin |
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Type Poster Presentation |
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Theme Probiotics and Prebiotics: Excellence in Science and Clinical Translation |
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Topic Development of Probiotic and Prebiotic Foods, Medical Foods, Supplements and Drugs |
Authors
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Main Author Bharat Bhushan1 |
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Presenting Author Vijendra Mishra1 |
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Co-Author Jayesh Ahire2 LMT Dicks2 Vijendra Mishra1 |
Authors' Institution
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Department / Institution / Country Basic and Applied Science / National Institute of Food Technology Entrepreneurship and Management / India1 Microbiology / Stellenbosch University / South Africa2 |
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Background and Rationale The inadequate food intake and/or unbalanced food habits rendered the persistence of riboflavin (vitamin B2 or B2) deficiency among developing and developed nations. Few reports have claimed for B2-producing capabilities of lactic acid bacteria (LAB) and further scope of food fortification to mitigate riboflavin deficiency |
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Objectives: Indicates the purpose of the study The objective of this study was to isolate novel riboflavin-producing Lactobacillus strains of human and fermented-foods origin. |
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Methodology: Describe pertinent experimental procedures A total of sixty-five samples including fermented milks (curd and lassi) and human feces were tested for isolation of lactobacilli. The genus level identification was performed on the basis of microscopic, biochemical and molecular tests. All Lactobacillus isolates were screened for riboflavin production potential using B2-free growth conditions. Selected isolates were undergone actual B2-production and lactobacilli growth supernatants were tested for detection and bioavailability of produced vitamin using Enterococcus faecalis MTCC2729 for microbiological assay |
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Results: Summarize the results of the research Out of 150 screened colonies, 68 isolates were identified as lactobacilli. Five isolates, out of 68, primarily showed growth potential in B2-free conditions and four out of selected five were later confirmed as B2-producer on the basis of lactobacilli-culture-supernatant-mediated supplemental growth of auxotrophic mutant in riboflavin-free growth conditions. Also, this test confirmed the bioavailability of produced vitamin in a biological system. Moreover, present report confirmed the preciseness of B2-free growth conditions as screening protocol for isolating riboflavin-producing lactobacilli. |
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Conclusions: State the main conclusions Present study showed the presence of riboflavin producing capability in both fecal and milk isolates. While the other functionalities of B2-producing strains have yet to be confirmed, the study adds on knowledge in bio-prospecting of vitamin-producing novel LAB strains. |
Requires Audio or Video system for Presentation?: No
